三维分析缺血性卒中大鼠内皮卒中后血管生成
染色方法：
荧光染料标记
标记方法：
lectin-FITC Lectin-DyLight 594
包埋方法：
树脂包埋
成像平台：
BioMapping 5000
Figure 1. Inhibiting Sema3E/PlexinD1 signal- ing improves vascular network recovery after tMCAO. A) Schematic illustration of treatment with oriented injection of LV-mediated Plex- inD1-specific shRNA (sh) or control shRNA into ischemic cortex. B) The representative reconstructed images of cerebral vascular network by MOST at d 14 after tMCAO. Original magnification value, 35. C) Blood vessel density of in perilesion area of recon- structed images was quantified. Data are presented as means 6 SEM ; n >= 3. *P , 0.05, ***P , 0.001 vs. control or indicated group.
Figure 1. Inhibiting Sema3E/PlexinD1 signal- ing improves vascular network recovery after tMCAO. A) Schematic illustration of treatment with oriented injection of LV-mediated Plex- inD1-specific shRNA (sh) or control shRNA into ischemic cortex. B) The representative reconstructed images of cerebral vascular network by MOST at d 14 after tMCAO. Original magnification value, 35. C) Blood vessel density of in perilesion area of recon- structed images was quantified. Data are presented as means 6 SEM ; n >= 3. *P , 0.05, ***P , 0.001 vs. control or indicated group.
2019年4月3日，华中科技大学同济医学院附属协和医院神经内科胡波教授课题组，使用MOST技术来充分展示包括毛细血管在内的血管模式的细节，揭示了在短暂的大脑中动脉闭塞后，抑制 PlexinD1信号导致缺血区血管网有组织地恢复的可能机制。文章发表在《FASEB》杂志。
参考文献
参考文献[1]:Zhou YF et al., Sema3E/PlexinD1 signaling inhibits postischemic angiogenesis by regulating endothelial DLL4 and filopodia formation in a rat model of ischemic stroke. FASEB 2019 Jan 17